Bid4thatjob.com

Bid 4 That Job.com

Looking for the right person for your home construction and renovation job has never been easier than today. This is because there is a great online auction for home construction site for you to use, called Bid4ThatJob.com. this is basically a site that works as platform for service providers and tradesmen to meet and get to know about the availability of projects and services from tradesmen.

This is a safer option not only for your posting your jobs, but also for choosing the right tradesman for your job. Posting a job on Bid4ThatJob.com is really easy. All you have to do is to fill up their online form with details of your job, preferably with preferable rates, date of completion and photos of the land or building where you need work to be done.

You are not obliged to accept quotes you receive

You are in no way obliged to accept all the quotes that you receive; you are at liberty of either rejecting all these quotes or of approaching a few probable prospects and finding out more about their experience and competence at completing your job.

If you don’t find the right person after posting your job offer for some time, you can also extend the offer for a longer stretch of time or if required, you can also withdraw the job offer from the site completely.

The greatest advantage of using the services of the site Bid4ThatJob.com is that you need not look high and low for the right person for your renovation and construction work, everything is done in the comfort of your home, with the help of your computer.

In fact, tradesmen instead come to you, offering their services to complete your job. With this, you not only save time but also money while looking for the right person for your job.

Furnish information of yourself, the tradesman

If you are a tradesman, all you have to do is to fill out the form found on the site asking for details of yourself and your experience. You have to furnish basic information about yourself like your contact information, your hourly or project rates, your area of expertise and other matters pertaining to home construction jobs.

Once you are registered on sites like Bid4ThatJob.com, you will be regularly sent emails about job offers that seem to fit your cap. It is up to you to look through these job offers and decide which job offer looks best for yourself.

Everything is done for free

An advantage of registering and getting work through sites like Bid4ThatJob.com is that you need not advertise about your services and look out for job opportunities. All this is done with a few clicks of your mouse, in the comfort of your home and office. Moreover, all this work is done for free, without having to pay any fees or money.

After seeing all the benefits that are offered by online auction sites, there should be no inhibitions of anyone on posting jobs, and on looking for jobs in these sties, while sitting in their comfortable homes.

Yellow Pages UAE - UAE’s best local search site

The site is nicely divided in to segments like; News, Mall Guide, UAE Articles, Yellow Pages Services & Useful Information… The same information is also available on mobile for free by just typing the WAP address: http://mobile.yellowpages.ae

About Yellow Pages Association:

The Yellow Pages Association (YPA) is the trade organization of a print and digital media Yellow Pages industry. Association members include Yellow Pages professional from around the world, including the industry’s international, national and local sales forces, certified marketing representatives (CMRs) and associate members, a group of industry stakeholders that include Yellow Pages advertisers, vendors and suppliers. The Yellow Pages Association has members in 29 countries.

Yellowpages.ae:

YellowPages.ae is UAE’s trusted source for local search information.

Al Wahda Express (AWE) is the official publisher of Etisalat Telephone Directories. Since 1986, AWE has been a leader in advertising and contact solutions through our flagship products, the Yellow Pages and White Pages directories. While maintaining a leading position in printed directories, the company has rapidly grown into one of the country’s most prolific providers of online advertising through www.yellowpages.ae. We are committed to keeping people and business - In Touch, wherever - whenever.

According to the site statistics as of now, there are 6852 users registered on

Yellow Pages UAE.

Author:

Asif Ahmed is an ardent reader & follower of all webmaster & internet news online. He is a frequent user of Yellow Pages UAE.

Revenue Cycle Management

Revenue cycle management is the hot topic for hospital managers today-the subject of countless seminars and articles, and the current watchword for consultants across the healthcare industry. Yet hospitals that have made the necessary operational and organizational changes to implement effective new revenue-cycle processes remain in the minority. Healthcare revenue cycle management is dependent on accurate information being in the right system at the right time. The reliance on manual data entry slow reimbursement and resulting errors can hinder payment employee workload. Truworth is a healthcare integration solution that gives hospitals and healthcare vendors the ability to automate revenue cycle tasks resulting in more accurate billing and faster reimbursements. From automating data entry to multi step processes involving decision making, Truworth Healthcare is used in thousands of ways. Hospitals are eliminating errors, speeding information and reducing costs through workflow automation.

Healthcare revenue cycles are being slowed down as organizations work with different vendors, insurance companies, government agencies and various third parties to register, verify, bill and collect revenues. Today, more and more directors of patient financial service departments face increasing challenges while trying to prevent employee morale from bottoming out during the myriad changes occurring in the healthcare landscape. Patient accounting personal are witnessing constant changes in how they are required to do their jobs. These changes are being fueled by many factors, including emerging technology, merges and acquisitions, downsizing, conversions to new billing systems, stringent claims filling deadlines, modifications to billing forms, and complex contracts for nearly all players. Reinventing whole processes yields beneficial and predictable outcomes with payoffs that are both tangible and intangible. Improving processes by creating Electronic managers; by ensuring the accurate flow of information and establishing immediate response and notifications can impact revenue streams, patient care, and hundreds of other initiatives.

TLR1- cluster of differentiation 281

Toll-like receptor 1 (TLR1) often designated as CD281 (cluster of differentiation 281), a member of the Toll-like receptor family recognizes pathogen-associated molecular pattern with specificity for gram-positive bacteria. TLR1 is a 786-residue type I transmembrane protein with a 581-amino acid leucine-rich extracellular domain (ECD), a 23-amino acid transmembrane domain (amino acids 582 to 604), and a 181-amino acid cytoplasmic Toll homology signalling domain (1, 2). TLR1 maps to chromosome 4p14 with a calculated molecular weight of 84 kDa (3, 4). TLR1 is most closely related to TLR6 and TLR10 with 68% and 48% overall amino acid sequence identity, respectively. Among members of the TLR family, TLR1 along with TLR6 comprise the most highly conserved pair and appear to have arisen more recently during evolution through a gene duplication event. Different length transcripts presumably resulting from use of alternative polyadenylation site, and/or from alternative splicing, have been noted for TLR4.

In vivo, two different sized transcripts for TLR1 are observed suggesting that the mRNA is alternatively spliced to generate two different forms of the protein. TLR1 mRNA is ubiquitously expressed and found at higher levels than the other TLRs. Of the major leukocyte populations, TLR1 is most highly expressed by monocytes, but is also expressed by macrophages, dendritic cells (DCs), polymorphonuclear leukocytes, B, T, and NK cells. While TLR1 expression is most significantly upregulated by autocrine IL-6, it is also elevated by IFN-γß, IL-10, and TNF-α. However, TLR1 level is unaffected by exposure to both Gram-positive and Gram-negative bacteria.

TLR1 along with TLR6 functions as a co-receptor for TLR2, which confers ligand specificity and enables cell signaling. Collectively, these receptor pairs mediate immune responses to a wide variety of acylated cell wall components derived from Gram-positive bacteria, Gram-negative bacteria, mycoplasma, spirochetes, and fungi. TLR1 also heterodimerizes with TLR4, not to enhance its function, but to inhibit TLR4 activity (5, 6). Defects in the TLR1/2 signaling pathway may account for human hyporesponsiveness to OspA vaccination.

Through the reciprocal exchange of extracellular domains between the human TLRs 1 and 6, it has been revealed that TLR1/2 and TLR2/6 receptor pairs exhibit different specificities toward many microbial agonists including diacylated and triacylated lipopeptides, which is determined by the region comprised of leucine-rich repeat motifs 9–12 of these receptors. A recent finding suggests that three nonsynonymous single nucleotide polymorphisms (SNPs) are located in this region of TLR1. A variant of TLR1 based upon the SNP P315L, located in the loop of LRR motif 11 (LRR11), is greatly impaired in mediating responses to lipopeptides. The P315L SNP may predispose certain individuals to infectious diseases for which the sensing of microbial cell components by TLR1 is critical to innate immune defense (7). Thus variation in the inflammatory response to bacterial lipopeptides is regulated by a common TLR1 transmembrane domain polymorphism that could potentially impact the innate immune response and clinical susceptibility to a wide spectrum of pathogens.

Reference:

1. Janeway, C. A., Jr, R. Medzhitov. 2002. Innate immune recognition. Annu. Rev. Immunol. 20: 197-216.

2. Takeda, K., T. Kaisho, S. Akira. 2003. Toll-like receptors. Annu. Rev. Immunol. 21: 335-376.

3.Beutler, B., Z. Jiang, P. Georgel, K. Crozat, B. Croker, S. Rutschmann, X. Du, K. Hoebe. 2006. Genetic analysis of host resistance: Toll-like receptor signaling and immunity at large. Annu. Rev. Immunol. 24: 353-389.

4. Rock, F.L. et al. (1998) Proc. Natl. Acad. Sci. USA 95:588.

5. Ozinsky, A. et al. (2000) Proc. Natl. Acad. Sci. USA 97:13766.

6. Wyllie, D.H. et al. (2000) J. Immunol. 165:7125.

7. Katherine O. Omueti The Journal of Immunology, 2007, 178: 6387-6394.

IMGENEX India Pvt Ltd. the only biotech company in Orissa and one of its kinds in Eastern India. IMGENEX India started in Oct as an outsourcing branch of IMGENEX Corporation, San Diego, USA.

TLR10- cluster of differentiation 290

Toll-like receptor 10 (TLR10) often known as CD290 (cluster of differentiation 290), is the most recently identified human homolog of the Drosophila TOLL protein. Human TLR10 is an orphan member of the Toll-like receptor family that recognizes pathogen-associated molecular pattern. Like other members of the TLR family, TLR10 contains a signal peptide, multiple leucine-rich repeats, a cysteine-rich domain, a transmembrane domain, and a cytoplasmic TOLL interleukin-1 receptor domain. The human TLR10 gene occupies 3,269 bases arranged in three exons on the short arm of chromosome 4 (4p14) and encodes an 811-amino acid protein, approximately 50% identical to TLR1 and to TLR6. TLR10 is most closely related to TLR1 and TLR6 with 48% and 46% overall amino acid identity, respectively. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene (1).

In vivo, TLR10 mRNA expression is highest in immune system-related tissues including spleen, lymph node, thymus, tonsil. TLR10 mRNA is most highly expressed on B cells. In vitro, TLR10 is moderately upregulated by autocrine IFN-γ, IL-1β, IL-6, IL-10, and TNF-α in PMA-differentiated THP-1 cells. TLR10 mRNA expression in THP-1 cells is elevated after exposure to both Gram-positive and Gram-negative bacteria. Ex vivo, monocyte TLR10 expression increases while granulocyte expression decreases on exposure to Gram-negative bacteria. (2, 3)

Due to absent of its rat homologue, the natural ligand for TLR10 has not been identified yet. Genomic studies indicate that TLR10 is in the same locus that contains TLR1 and TLR6 and are also structurally similar to each other. It has been speculated that, like TLR1 and TLR6, TLR10 may form a heterodimer with TLR2 and thereby be sensitive to similar pathogen-associated molecular patterns (PAMPs). Recent studies have shown that TLR10 was not only able to homodimerize but also heterodimerize with TLRs 1 and 2. (1)

TLR10 has been identified as a potential asthma candidate gene because early life innate immune responses to ubiquitous inhaled allergens and PAMPs may influence asthma susceptibility and thus TLR10 genetic variation may often contributes to asthma risk. (4)

References:

1. The Journal of Immunology, 2005, 174: 2942-2950.

2. Chuang, T. & R.J. Ulevitch (2001) Biochim. Biophys. Acta 1518:157.

3. Zarember, K.A. & P.J. Godowski (2002) J. Immunol. 168:554.

4. Hornung, V. et al. (2002) J. Immunol. 168:4531.

IMGENEX India Pvt Ltd. the only biotech company in Orissa and one of its kinds in Eastern India. IMGENEX India started in Oct as an outsourcing branch of IMGENEX Corporation, San Diego, USA.

In Search of Putative FOXP3+ Cell Surface Markers

Despite intense interest and scrutiny focused on FOXP3 as a key protein & master transcription factor, isolating and enriching for viable FOXP3 positive cells remains a challenge. Although cell separation/staining via CD4+CD25+ selection is commonly used, this technique has limited applications. Thus, surface markers specific for FOXP3 positive cells would be invaluable research tools as they would:

• Facilitate isolation & purification of viable

Treg cells

• Distinguish naturally occurring

CD4+CD25+cells from both naive and

recently activated CD4+CD25-

nonregulatory T cells

• Allow therapeutic manipulation of

Treg cells

As the search for putative FOXP3+ markers continue, Neuropilin-1, GPR83, & FR4 have emerged as potential candidates. IMGENEX is excited to offer a panel of flow cytometric characterized antibodies against:

• Neuropilin-1 (clone 211H6.01)

• GPR83 (polyclonal)

• Folate Receptor 4 (clones 12A5 & TH6)

Flow cytometric analysis of intracellular FOXP3 (IMG-5802D) and cell surface FR4 with clone 12A5 (IMG-6217C) (left) and clone TH6 (IMG-6218C) (right) at 0.06 ug/10^6 mouse splenocytes.

Flow cytometric analysis of Neuropilin-1 in CD4+CD25+ human PBMCs using A) an isotype control & B) DDX0440 at 0.5 ug/10^6 cells. These antibodies are available in multiple sizes and conjugates.

IMGENEX India Pvt Ltd. the only biotech company in Orissa and one of its kinds in Eastern India. IMGENEX India started in Oct as an outsourcing branch of IMGENEX Corporation, San Diego, USA.

A Functional Contributor to Cancer Metastatic Potential

Tumor metastasis plays a key role in assessing treatment strategies and an important factor in determining patient prognosis. Not surprisingly, significant effort has been focused on elucidating the molecular basis behind tumor metastasis. This understanding may allow development of diagnostic and prognostic tools and likely provide novel therapeutic targets.

In a recent study by Crawford et al. published in PNAS, it was shown that Brd4, a ubiquitously expressed 200-kDa nuclear protein broadly expressed in many tissues, significantly reduced tumor growth and metastasis when implanted into mice. Further, Microarray analysis performed by the same group identified a correlation between Brd4 activation and disease progression/patient survival.

Together, this evidence strongly suggests that Brd4 plays a key role in breast cancer progression and an underlying mechanism of many metastasis-predictive gene signatures.

Although it remains to be determined if Brd4’s role is that of a proximal factor or an intermediary molecule of some other inherited factor that drives the progression of breast cancer, its functional importance is emerging as both a diagnostic and prognostic tool with therapeutic significance.

IMGENEX India Pvt Ltd. the only biotech company in Orissa and one of its kinds in Eastern India. IMGENEX India started in Oct as an outsourcing branch of IMGENEX Corporation, San Diego, USA.

Neuropilin-1/BDCA-4/CD304

Neuropilin-1 (Nrp-1) was recently identified as a possible surface marker for naturally occurring CD4+CD25+ regulatory T cells and is constitutively expressed on the cell irrespective of its activation status. Nrp-1 is a type 1 membrane protein with three unique functions. In neuronal cells, Nrp-1 binds the class 3 semaphorins, which are neuronal chemorepellents, and plays a role in the directional guidance of axons. It also form complexes with the plexinA subfamily members and mediate the semaphorin-elicited inhibitory signals into neurons. Moreover in endothelial cells, Nrp-1 binds a potent endothelial cell mitogen, vascular endothelial growth factor (VEGF)165 and thus regulates vessel formation (1). The protein is also described to mediate homophilic interactions between dendritic cells and T cells in human, which confirms its role in immune function. Amongst T cells, Nrp-1 is preferentially expressed on T regulatory cells (Tregs) (2). It has been suggested that Neuropilin-1 acts as glue between Tregs and dendritic Cells (4). Neuropilin-1 expression on Tregs has been shown to enhance their interactions with immature dendritic cells (iDCs) during antigen recognition (4).

Treg cells are potential components of the immune system controlling immune responsiveness to self and alloantigens. Apart from the expression of the two surface molecules, CD4 and CD25, Treg cells are also known to express the transcription factor Foxp3, which is essential for the development and function of Treg cells. Recently, several cell surface molecules, such as, CTLA-4, GITR, LAG3, GPR83, Folate receptor 4 have been shown to be expressed at high level on regulatory T cells. Besides the currently known other cell surface receptors for Treg cells such as the B7-family members PD1, and ICOS, are also up regulated in activated non-regulatory CD4+T cells.

Recent research works to identify a specific cell surface marker for Tregs has led to identification of Nrp-1 as a cell surface molecule that is expressed by 80% of CD4+CD25+ Tregs but not on naïve cells. Further studies reveal the expression of the Nrp-1 gene to be regulated by the transcription factor Foxp3. Ectotopic expression of FoxP3 in CD4+ T cells lead to up regulation of Nrp-1 and increases interaction time between Tregs and iDCs, resulting in higher sensitivity to limiting amounts of antigens (5). Anti-Nrp-1 antibody treatment interferes with interactions between Tregs and iDCs. Moreover the expression of the protein is also significantly low in naive non-regulatory T cells (CD4+CD25- T cells) and is further down regulated after TCR stimulation. Thus Nrp-1 represents a novel surface marker on Treg cells with several important functions.

IMGENEX India Pvt Ltd. the only biotech company in Orissa and one of its kinds in Eastern India. IMGENEX India started in Oct as an outsourcing branch of IMGENEX Corporation, San Diego, USA.

Folate Receptor 4 (FR4)

Folates play an important role in protein and nucleic acid biosynthesis and are particularly recognized for their roles in spinal canal and brain development during early pregnancy. FR4 is expressed at high levels on both natural and TGF-b induced Tregs. The majority of CD4+ Tregs express FR4 along with CD25 and FoxP3. FR4 antibodies (e.g. clone 12A5 and TH6) recognize a subtype of the receptor (35kD) for the vitamin folic acid also known as folate receptor d, and folate-binding protein 3. Additionally, there are functional similarities in regulating immune responses between FoxP3 and FR4 expressing CD4+/CD25+ Tregs. CD4+CD25+ FR4hi cells may be used as a functional immunosuppressor Treg population which would be expected to express FoxP3. Accordingly, anti-FR4 antibodies may be useful for cell surface staining of Tregs and easy isolation by FACS or magnetic bead separation while maintaining the cells viability for subsequent down-stream applications.

Key Publication Findings

• FR4 appears to be constitutively expressed in Treg cells at a higher level than other activated or naive T cells

• Not simply a marker for natural Tregs, but functionally essential.

IMGENEX India Pvt Ltd. the only biotech company in Orissa and one of its kinds in Eastern India. IMGENEX India started in Oct as an outsourcing branch of IMGENEX Corporation, San Diego, USA.

TLR2- cluster of differentiation 282

Toll-like receptor 2 (TLR2), often designated as CD282 (cluster of differentiation 282) is a type I transmembrane protein belonging to the large homologous family of Toll like receptors. TLR2 acts as functional receptor for both Gram-positive and Gram-negative bacteria. Like all other members of the TLR family, TLR2 is composed of an extracellular domain containing multiple leucine-rich repeats (LRRs), a transmembrane region, and a cytoplasmic tail containing the conserved TIR domain. TLR2 maps to chromosome 4q31-32 and encodes a putative 784 amino acid protein with 19 N-terminal LLRs and a calculated molecular weight of 84 kDa (1, 2, 3). Comparison of the amino acid sequence reveals that TLR2, TLR1, and TLR6 form a TLR subfamily, which presumably diverged from one common ancestral gene. In humans, TLR10 is also a member of this TLR2 subfamily. Among all TLR, TLR1 and TLR6 have the highest identity of overall amino acid sequence, which is 66%, and a similar genomic structure and thus it is assumed that they are the evolutionary products of gene duplication.

In vivo transcripts for TLR2 are observed suggesting that the mRNA is alternatively spliced. TLR2 mRNA expression is observed in brain, heart, lung, and spleen tissues and is highest in PBLs, specifically those of myelomonocytic origin. In vitro PMA-differentiated THP-1, TLR2 is most significantly upregulated by autocrine IL-6 and TNF-α, IL-1β, and IL-10. Further, TLR2 mRNA expression is elevated after exposure to both Gram-positive and Gram-negative bacteria. The increase in TLR2 expression in monocytes and granulocytes on exposure to Gram-negative bacteria is only very modest. Furthermore, TLR2 appears to be up-regulated on mononuclear cells during disorders such as chronic obstructive pulmonary disease, influenza virus infections, and sepsis

TLR2 act as signal transducers for various bacterial components which include lipoproteins derived from M. tuberculosis, Borrelia burgdorfei, Treponema pallidium and Mycoplasma fermentans. In addition, TLR2 mediates cellular responses to a wide variety of infectious pathogens and their products which include yeast cell walls, whole mycobacteria, mycobacterial ara-lipoarabinomannan, whole Gram-positive bacteria, peptidoglycan (PGN), Treponema glycolipid and Trypanosoma cruzi glycophosphatidylinositol anchor. TLR2 forms heterodimers with TLR1, TLR6 and possibly TLR10, where each complex is particularly sensitive to subsets of TLR2-associated pathogen-associated molecular patterns (PAMPs). It has been studied that TLR6 and TLR2 function together to detect Gram-positive bacteria, PGN and zymosan, whereas TLR2 functions either alone or with TLRs other than TLR6 to detect bacterial lipopeptides. More recent studies have suggested that, like TLR4, TLR2 complexes require CD14 and MD-2 for detection of PAMPs and signaling. (4, 5) Upon ligand recognition, TLR2 recruits both the TIR domain-containing sorting adaptor TIRAP and the signaling adaptor MyD88, and initiates the MyD88-dependent pathway. The MyD88-dependent pathway activates nuclear factor (NF)-κB, activator protein-1 (AP-1) and interferon regulatory factor 5 (IRF5), which induce inflammatory cytokine expression such as IL-6, IL-12, and TNFα. (6)

Aside from detection of non-self patterns, TLR2 complexes are also capable of detecting altered self patterns, such as those displayed by necrotic cells. Further, recent evidence indicates that TLR2 is recruited to phagosomes and may be directly involved in the internalization of microbial products by cells.

Reference:

1. Rock, F.L. et al. (1998) Proc. Natl. Acad. Sci. USA 95:588.

2. Chaudhary, P.M. et al. (1998) Blood 91:4020.

3. Dunne, A. & L.A.J. O’Neill (2003) Sci. STKE 2003:re3.

4. Modlin, R.L. (2002) Ann. Allergy Asthma Immunol. 88:543.

5. J Endotoxin Res. 2000;6(5):401-5

6. Annual Review of Biochemistry Vol. 76: 447-480 (Publication date July 2007)

IMGENEX India Pvt Ltd. the only biotech company in Orissa and one of its kinds in Eastern India. IMGENEX India started in Oct as an outsourcing branch of IMGENEX Corporation, San Diego, USA.